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Presence of Helicobacter pylori in the oral cavity, oesophagus, stomach and faeces of patients with gastritis

Identifieur interne : 00A717 ( Main/Exploration ); précédent : 00A716; suivant : 00A718

Presence of Helicobacter pylori in the oral cavity, oesophagus, stomach and faeces of patients with gastritis

Auteurs : F. Namavar [Pays-Bas] ; R. Roosendaal [Pays-Bas] ; E. J. Kuipers [Pays-Bas] ; P. De Groot [Pays-Bas] ; M. W. Van Der Bijl [Pays-Bas] ; A. S. Pe A [Pays-Bas] ; J. De Graaff [Pays-Bas]

Source :

RBID : ISTEX:672FC03624FA0062439D424A1C55D82B2C97603F

English descriptors

Abstract

Abstract: The presence ofHelicobacter pylori in the oral cavity (6 sites), oesophagus, stomach and bowel of 20 dyspeptic patients was investigated. Samples were cultured on three selective media and analyzed by 16S rDNA polymerase chain reaction (PCR) and southern hybridization.Helicobacter pylori DNA was detected by PCR from oral-cavity samples of three (20 %) and from faeces samples of only one (7 %) of the patients whose stomach biopsies were positive forHelicobacter pylori. When culture was used, the microorganism's rate of recovery from the oral cavity and faeces was 13 % and 7%, respectively. One patient had aHelicobacter pylori-like organism in samples collected from the tongue and palate. Both strains were urease, catalase and oxidase positive and grew microaerophilically but were negative on PCR analysis. This demonstrates the possibility of false identification ofHelicobacter pylori by use of routine enzyme reactions. Interestingly, specimens collected from the cheeks of three patients were positive forHelicobacter pylori by PCR analysis. This is the first instance of detection of this micro-organism in the cheek.

Url:
DOI: 10.1007/BF02310363


Affiliations:


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Le document en format XML

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<div type="abstract" xml:lang="en">Abstract: The presence ofHelicobacter pylori in the oral cavity (6 sites), oesophagus, stomach and bowel of 20 dyspeptic patients was investigated. Samples were cultured on three selective media and analyzed by 16S rDNA polymerase chain reaction (PCR) and southern hybridization.Helicobacter pylori DNA was detected by PCR from oral-cavity samples of three (20 %) and from faeces samples of only one (7 %) of the patients whose stomach biopsies were positive forHelicobacter pylori. When culture was used, the microorganism's rate of recovery from the oral cavity and faeces was 13 % and 7%, respectively. One patient had aHelicobacter pylori-like organism in samples collected from the tongue and palate. Both strains were urease, catalase and oxidase positive and grew microaerophilically but were negative on PCR analysis. This demonstrates the possibility of false identification ofHelicobacter pylori by use of routine enzyme reactions. Interestingly, specimens collected from the cheeks of three patients were positive forHelicobacter pylori by PCR analysis. This is the first instance of detection of this micro-organism in the cheek.</div>
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